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10.1109/EMBC44109.2020.9176843 Annu

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Over the last two and half decades, many efforts have been made on developing and implanting in vitro protocols to successfully differentiate hPSCs into insulin-producing -cells with key features of bona fide mature -like cells using multi-stages directed differentiation protocols that recapitulate and phenocopy all specific stages of pancreas embryogenesis ( However, many protocols developed to differentiate stem cells into insulin-expressing -cells in vitro have faced a roadblock: the resulting -cells often exhibit an immature phenotype with impaired glucose-stimulated insulin secretion (GSIS) compared to cadaveric islets